Brozyna, M.; Stepnicka, Z.; Tyminska, N.; Dudek, B.; Kapczynska, K.; Matkowski, A.; Sun, Y.; Junka, A.

The rising interest in essential oils (EOs) as antimicrobial agents demands evaluation frameworks that move beyond anecdotal efficacy and toward structured, reproducible assessment. In this study, we examined the strain-dependent response of Pseudomonas aeruginosa to Pharmacopoeia-grade Thyme Essential Oil (TEO) or polyhexamethylene biguanide antiseptic (PHMB), using a panel of ten genetically diversified strains in planktonic and biofilm form and by complementary in vitro models. Despite uniform test conditions, we observed striking inter-strain variability: TEO Minimal Inhibitory Concentrations (MICs) differed by up to 1000-fold, and biofilm susceptibility profiles ranged from full tolerance to near-complete eradication. Notably, strains with low metabolic activity and sparse cell populations-but high matrix biomass-exhibited reduced responsiveness to TEO, while susceptibility to PHMB was more consistent, though not absolute. These findings highlight the critical influence of both microbial phenotype and agent formulation on antimicrobial outcomes. Rather than framing EOs as superior or inferior alternatives, our results advocate for their integration into a stewardship paradigm-one that values standardization, model-based evaluation, and informed formulation. In this context, we position essential oil stewardship not as a constraint, but as a necessary evolution for their credible inclusion in antimicrobial strategies.