Higgs, V.; Anwas, D.; Astapenka, A.; Toro-Tapia, G.; Das, R.

Neurons possess a highly polarised morphology, established through axon formation. However, the mechanisms regulating stable axon initiation during embryonic development remain poorly understood. Here, using fixed-tissue super-resolution and high-resolution live-tissue imaging in developing chick spinal cord, we demonstrate that the multifunctional G-protein Gi2 coordinates the interconnected neurofilament and microtubule networks to achieve stable axon outgrowth. Before axon initiation, microtubule network orientation shifts towards the site of the future axon where coiled Gi2-associated neurofilaments accumulate asymmetrically before unfurling into the initiating axon behind microtubules. Crucially, Gi2 is associated with neurofilaments and microtubules at points of contact. Gi2 depletion reduces engagement between these cytoskeletal networks, and leads to impaired passage of neurofilaments into initiating axons and disrupted axon outgrowth, supporting a role for Gi2 in regulating microtubule-driven incorporation of neurofilaments in nascent axons. These findings advance mechanistic understanding of polarity establishment and offer an enriched view of cytoskeletal regulation during axon formation.