Hayden, C. M. T.; Arieta, L. R.; Copeland, J. M.; Busa, M. A.

With metabolic disease on the rise across the globe, the devices that can provide precise and reliable estimates of energy expenditure and macronutrient oxidation can play a critical role in the development and evaluation of therapeutic regimes and wearable technologies that can be used outside of the laboratory. Whereas, metabolic carts can provide short-term (minutes to hours) metabolic measurements, whole-room calorimeters enable long-duration (hours to days) metabolic assessment, providing insights into how metabolism changes in response to meals, activity, sleep, etc. Obtaining accurate metabolic measurement via whole-room calorimetry, however, requires rigorous methods for calibration and quality assurance. To date most room calorimeters have been tuned to assess energy expenditure over long periods of time, i.e. 24-hours. Here we present novel calibration and signal processing techniques and recommendations that aim to improve the utility of metabolic chambers for use over different measurement epochs. This work serves as both a transparent description of our hardware, validation procedures, and data processing approaches.